Journal: Molecular Medicine Reports
Article Title: Puerarin attenuates myocardial ischemia-reperfusion injury by inhibiting myocardium pyroptosis via the NRF2/HO-1 signaling pathway
doi: 10.3892/mmr.2026.13824
Figure Lengend Snippet: Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) Hoechst 33342/PI staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
Article Snippet: The Hoechst 33342/PI double fluorescence staining kit (cat. no. CA1120; Beijing Solarbio Science & Technology Co., Ltd.) was used to detect pyroptosis in H9C2 cardiomyocytes from each group.
Techniques: Staining, Western Blot, Expressing, Over Expression, Negative Control, Binding Assay